Ida Bagus Gede Darmayasa : “ Trichoderma asperellum TKD potential to inhibit the growth of Aspergillus flavus FNCC6109 as efforts to reduce contaminants aflatoxins B1 in feed concentrate “

Monday, October 11, 2015.  Graduate Program held another open session on behalf of the Doctoral Promotion Promovendus Drs. Ida Bagus Gede Darmayasa, M.Si. From the Livestock Doctoral Program with a dissertation entitled “ Trichoderma asperellum TKD potential to inhibit the growth of Aspergillus flavus FNCC6109 as efforts to reduce contaminants aflatoxins B1 in feed concentrate “.The event was chaired by Prof. trial Dr. dr. A A. Raka Sudewi, Sp.S (K).

In his dissertation stated that Aspergillus flavus is a fungus that contaminates concentrate feed frequently. This fungus is able to produce aflatoxin B1 that are harmful to animals and humans Identification  of  Trichoderma sp. TKD had done with  macroscopic,  microscopic and molecular using internal transcribed spacer region (ITS 5 F: 5'

GGAAGTAAAAGTCGTAACAAGG-3 'and 4 R LTS: 5'- TCCTCCGCTTATTGATATGC-3'). inhibition and filtrate potential of Trichoderma sp. TKD against A. flavusFNCC61 09 in vitro is done by using the dual culture method and diffusion wells.Meanwhile in the vivo tests, conducted on a model that added the filtrate concentrate feed Trichoderma sp. TKD with the concentration of 3g / 100g; 6g / 100g and 9g / 100g. In the “in vivo” test parameters are measured is the population of A. flavus FNCC6109 using plating method and content of aflatoxin BJ was done by using ELISA (Enzyme Linked lmmuno Sorbent Assay). Type of secondary metabolites in the filtrate was analyzed by GC-MS (Gas Chromatography Mass Spectrometry QP2010 ultra Shimadzui). Filtrate toxicity of Trichoderma sp. TKD tested on mice (Mus musculus Lstrain Balb / c) to observe the liver function through measurement of AST, ALT and creatinine using the reagent Fluitest GOT ASA T starter, reagent Fluitesi GPT ASA T starter, reagent Fluitest creatinine were analyzed by AMS (Analyzer Medical System BOECO Germany), as well as liver and kidney biopsies using HE staining.

The results showed Trichoderma sp, TKD isolated from the rhizosphere of maize (Zea mays) identified as Trichoderma asperellum and has the potential to inhibit the growth of A. f1avusFNCC6109 of 98.849 ± 1.100. Identified 10 types of secondary metabolites were found in fraction IV filtrate T. asperellum TKD namely: dl- Mevalonic acid lactone; 3-Hexadecene,(Z)-(CAS); Tetradecane; Phenol, 3,5- bis(J .l-dimethylethyl); Dodecanoicanoic acid, methyl ester(CAS)methyl laurate; 3-Eicosene(E)-(CAS), Hexadecane; 2-Undecene, 3-methyl-,{Z}-; 3- Eicosine.tli)- dan Hexadecanoic acid, methyl ester (CAS)methyl palmitate. There is no acute toxicity in mice given the concentration of 9 / 100g filtrate T. asperellum TKD. SGOT, SGPT. (ari)